Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Applications in Bacteriology: Brazilian contributions

Authors

  • Flávia Lúcia Piffano Costa Pellegrino Unidade de Farmácia, Centro Universitário Estadual da Zona Oeste-UEZO.
  • Thiago Pavoni Gomes Chagas Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense-UFF.
  • Maria Silvana Alves Laboratório de Bioatividade Celular e Molecular, Centro de Pesquisas Farmacêuticas, Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora-UFJF.
  • Ana Paula D’Alincourt Carvalho-Assef Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
  • Alexander Chapeaurouge Laboratório de Toxinologia, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, Brazil.
  • Marise Dutra Asensi Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.

DOI:

https://doi.org/10.34019/1982-8047.2017.v43.2859

Keywords:

Ciencias da saude

Abstract

Among its innumerous applications in Bacteriology, the Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) technique is evolving as a powerful tool for bacterial identification and antimicrobial resistance investigation. Publications have evaluated the MALDI-TOF MS performance in the identification of a series of bacterial pathogens, including the most common severe infectious agents, emergent pathogens involved with outbreaks of healthcare-associated infections, rare pathogens, and those whose isolation in culture media is difficult. As compared to conventional methods of bacterial identification, MALDI-TOF MS has proven to be a fast, accurate and cost-effective technique. Currently, MALDI-TOF MS has been used in antimicrobial resistance studies, since it has shown to be an efficient tool in detecting specific resistance mechanisms in bacteria, such as beta-lactamases production, for example. Here, we describe the advances in this growing field of mass spectrometry applied to Bacteriology, including Brazilian contributions.

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Author Biography

Flávia Lúcia Piffano Costa Pellegrino, Unidade de Farmácia, Centro Universitário Estadual da Zona Oeste-UEZO.

*Corresponding author: Profa. Dra. Flávia Lúcia Piffano Costa Pellegrino. Centro Universitário Estadual da Zona Oeste-UEZO. Av. Manuel Caldeira de Alvarenga, 1203. Campo Grande. Rio de Janeiro. Brazil. Zip code. 23070-200. Phone. 55.21.23326961. Email. flpellegrino@hotmail.com

Flávia Lúcia P. C. Pellegrino é biomédica graduada em 1998 pela Universidade Federal do Estado do Rio de Janeiro (UNIRIO). Possui mestrado (concluído em 2001) e doutorado (concluído em 2005) em Ciências/Microbiologia pelo Instituto de Microbiologia Paulo de Góes (IMPG) da Universidade Federal do Rio de Janeiro (UFRJ). Desenvolveu a parte experimental do doutorado na University of California, Berkeley, EUA (2002 e 2004). Possui dois títulos de pós-doutorado obtidos em 2006 e em 2001 na UFRJ. Tem experiência na área de Microbiologia Clínica com ênfase no estudo da Resistência bacteriana aos antimicrobianos e Epidemiologia molecular de patógenos bacterianos. Atualmente ocupa o cargo de professora adjunta de Microbiologia e Imunologia Clínicas do curso de Farmácia da UEZO (Centro Universitário Estadual da Zona Oeste) e colabora em estudos de pesquisa no Laboratório de Pesquisa em Infecção Hospitalar (LAPIH) do Instituto Oswaldo Cruz.

 

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Published

2018-10-15

How to Cite

1.
Pellegrino FLPC, Chagas TPG, Alves MS, Carvalho-Assef APD, Chapeaurouge A, Asensi MD. Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Applications in Bacteriology: Brazilian contributions. HU Rev [Internet]. 2018Oct.15 [cited 2024Nov.22];43(3):285-94. Available from: https://periodicos.ufjf.br/index.php/hurevista/article/view/2859

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Vol. 43 No. 3 (2017)

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Artigos de Revisão da Literatura

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