Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Applications in Bacteriology: brazilian contributions

  • Flávia Lúcia Piffano Costa Pellegrino Unidade de Farmácia, Centro Universitário Estadual da Zona Oeste-UEZO.
  • Thiago Pavoni Gomes Chagas Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense-UFF.
  • Maria Silvana Alves Laboratório de Bioatividade Celular e Molecular, Centro de Pesquisas Farmacêuticas, Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora-UFJF.
  • Ana Paula D’Alincourt Carvalho-Assef Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
  • Alexander Chapeaurouge Laboratório de Toxinologia, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, Brazil.
  • Marise Dutra Asensi Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Palavras-chave: Ciencias da saude

Resumo

Among its innumerous applications in Bacteriology, the Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) technique is evolving as a powerful tool for bacterial identification and antimicrobial resistance investigation. Publications have evaluated the MALDI-TOF MS performance in the identification of a series of bacterial pathogens, including the most common severe infectious agents, emergent pathogens involved with outbreaks of healthcare-associated infections, rare pathogens, and those whose isolation in culture media is difficult. As compared to conventional methods of bacterial identification, MALDI-TOF MS has proven to be a fast, accurate and cost-effective technique. Currently, MALDI-TOF MS has been used in antimicrobial resistance studies, since it has shown to be an efficient tool in detecting specific resistance mechanisms in bacteria, such as beta-lactamases production, for example. Here, we describe the advances in this growing field of mass spectrometry applied to Bacteriology, including Brazilian contributions.

Biografia do Autor

Flávia Lúcia Piffano Costa Pellegrino, Unidade de Farmácia, Centro Universitário Estadual da Zona Oeste-UEZO.

*Corresponding author: Profa. Dra. Flávia Lúcia Piffano Costa Pellegrino. Centro Universitário Estadual da Zona Oeste-UEZO. Av. Manuel Caldeira de Alvarenga, 1203. Campo Grande. Rio de Janeiro. Brazil. Zip code. 23070-200. Phone. 55.21.23326961. Email. flpellegrino@hotmail.com

Flávia Lúcia P. C. Pellegrino é biomédica graduada em 1998 pela Universidade Federal do Estado do Rio de Janeiro (UNIRIO). Possui mestrado (concluído em 2001) e doutorado (concluído em 2005) em Ciências/Microbiologia pelo Instituto de Microbiologia Paulo de Góes (IMPG) da Universidade Federal do Rio de Janeiro (UFRJ). Desenvolveu a parte experimental do doutorado na University of California, Berkeley, EUA (2002 e 2004). Possui dois títulos de pós-doutorado obtidos em 2006 e em 2001 na UFRJ. Tem experiência na área de Microbiologia Clínica com ênfase no estudo da Resistência bacteriana aos antimicrobianos e Epidemiologia molecular de patógenos bacterianos. Atualmente ocupa o cargo de professora adjunta de Microbiologia e Imunologia Clínicas do curso de Farmácia da UEZO (Centro Universitário Estadual da Zona Oeste) e colabora em estudos de pesquisa no Laboratório de Pesquisa em Infecção Hospitalar (LAPIH) do Instituto Oswaldo Cruz.

 

Referências

BARBERINO, M. G. et al. Direct identification from positive blood broth culture by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). Brazilian Journal of Infectious Diseases, v. 21, n. 3, p. 339-342, may./jun. 2017.

BIBERG, C. A. et al. KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil. Brazilian Journal of Microbiology, v. 46, n. 2, p. 501-504, jun. 2015.

BONIN, R. F. et al. Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach. Proteomics Clinical Applications, v. 8, n. 11-12, p. 916-923, dec. 2014.

BURCKHARDT, I.; ZIMMERMANN, S. Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry to detect carbapenem resistance within 1 to 2.5 hours. Journal of Clinical Microbiology, v. 49, n. 9, p. 3321-3324, sep. 2011.

BURILLO, A. et al. Gram-stain plus MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry) for a rapid diagnosis of urinary tract infection. PLoS ONE, v. 9, n. 1, p. e86915, jan. 2014.

CABROLIER, N. et al. Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry identifies Pseudomonas aeruginosa high-risk clones. Journal of Clinical Microbiology, v. 53, n. 4, p. 1395-1398, apr. 2015.

CARVALHAES, C. G. et al. Detection of SPM-1-producing Pseudomonas aeruginosa and class D--lactamase-producing Acinetobacter baumannii isolates by use of Liquid Chromatography-Mass Spectrometry and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Journal of Clinical Microbiology, v. 51, n. 1, p. 287-290, jan. 2013.

CARVALHAES, C. G. et al. Detection of carbapenemase activity directly from blood culture vials using MALDI-TOF MS: a quick answer for the right decision. Journal of Antimicrobial and Chemotherapy, v. 69, n. 8, p. 2132-2136, aug. 2014.

CHAN, W. S. et al. Complementary use of MALDI-TOF MS and real-time PCR-melt curve analysis for rapid identification of methicillin-resistant staphylococci and VRE. Journal of Antimicrobial and Chemotherapy, v. 70, n. 2, p. 441-447, feb. 2015.

CHERKAOUI, A. et al. Comparison of two Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. Journal of Clinical Microbiology, v. 48, n. 4, p. 1169-1175, apr. 2010.

CLARK, A. E. et al. Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: a fundamental shift in the routine practice of clinical microbiology. Clinical Microbiology Reviews, v. 26, n. 3, p. 547-603, jul. 2013.

COX, C. R. et al. Strain-level bacterial identification by CeO2-catalyzed MALDI-TOF MS fatty acid analysis and comparison to commercial protein-based methods. Scientific Reports, v. 5, n. x, p. 10470, jul. 2015.

DUBOIS, D. et al. Identification of a variety of Staphylococcus species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Journal of Clinical Microbiology, v. 48, n. 3, p. 941-945, mar. 2010.

FARINA, C. et al. Direct identification of microorganisms from positive blood cultures using the lysis-filtration technique and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): a multicentre study. New Microbiologica, v. 38, n. 2, p. 245-250, apr. 2015.

FEHLBERG, L. C. et al. Performance of MALDI-TOF MS for species identification of Burkholderia cepacia complex clinical isolates. Diagnostic Microbiology and Infectious Disease, v. 77, n. 2, p. 126-128, oct. 2013.

FERREIRA, L. et al. Microorganisms direct identification from blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Clinical Microbiology and Infection, v. 17, n. 4, p. 546-551, apr. 2011a.

FERREIRA, L. et al. Rapid method for direct identification of bacteria in urine and blood culture samples by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: intact cell vs. extraction method. Clinical Microbiology and Infection, v. 17, n. 7, p. 1007-1012, jul. 2011b.

FRICKMANN, H. et al. Rapid discrimination of Haemophilus influenzae, H. parainfluenzae, and H. haemolyticus by fluorescence in situ hybridization (FISH) and two Matrix-Assisted Laser-Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) platforms. PLoS ONE, v. 8, n. 4, p. e63222, apr. 2013.

GRIFFIN, P. M. et al. Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry to identify vancomycin-resistant enterococci and investigate the epidemiology of an outbreak. Journal of Clinical Microbiology, v. 50, n. 9, p. 2918-31, sep. 2012.

GROSSE-HERRENTHEY, A. et al. Challenging the problem of clostridial identification with matrix-assisted laser desorption and ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Anaerobe, v. 14, n. 4, p. 242-249, oct. 2008.

HOMEM DE MELLO DE SOUZA, H. A. et al. MALDI-TOF: a useful tool for laboratory identification of uncommon glucose non-fermenting Gram-negative bacteria associated with cystic fibrosis. Journal of Medical Microbiology, v. 63, n. 9, p. 1148-1153, sep. 2014.

HRÁBAK, J.; CHUDÁCKOVÁ, E.; WALKOVÁ, R. Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry for detection of antibiotic resistance mechanisms: from research to routine diagnosis. Clinical Microbiology Reviews, v. 26, n. 1, p. 103-114, jan. 2013.

ILINA, E. N. et al. Direct bacterial profiling by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry for identification of pathogenic Neisseria. Journal of Molecular Diagnostics, v. 11, n. 1, p. 75-86, jan. 2009.

JAMAL, W.; SALEEM, R.; ROTIMI, V. O. Rapid identification of pathogens directly from blood culture bottles by Bruker matrix-assisted laser desorption laser ionization-time of flight mass spectrometry versus routine methods. Diagnostic Microbiology and Infectious Disease, v. 76, n. 4, p. 404-408, aug. 2013.

JAMAL, W.; ALBERT, J. M.; ROTIMI, V. O. Real-time comparative evaluation of bioMérieux VITEK MS versus Bruker Microflex MS matrix-assisted laser desorption-ionization time-of-flight mass spectrometry systems, for identification of clinically significant bacteria. BMC Microbiology, v. 14, n. x, p. 289, nov. 2014.

JO, S. J. et al. Direct identification and antimicrobial susceptibility testing of bacteria from positive blood culture bottles by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and the Vitek 2 system. Annals of Laboratory Medicine, v. 36, n. 2, p. 117-123, mar. 2016.

JUNG, J. S. et al. Rapid detection of antibiotic resistance based on mass spectrometry and stable isotopes. European Journal of Clinical Microbiology & Infectious Diseases, v. 33, n. 6., p. 949-955, dec. 2014.

KEMPF, M. et al. Rapid detection of carbapenem resistance in Acinetobacter baumannii using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. PLoS ONE, v. 7, n. 2, p. e31676, feb. 2012.

KOK, J. et al. Identification of bacteria in blood culture broths using Matrix-Assisted Laser Desorption Ionization Sensityper™ and Time of Flight Mass Spectrometry. PLoS ONE, v. 6, n. 8, p. e23285, aug. 2011.

LA SCOLA, B.; RAOULT, D. Direct identification of bacteria in positive blood culture bottles by Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry. PLoS ONE, v. 4, n. 11, p. e8041, nov. 2009.

LAVIGNE, J-P. et al. Mass spectrometry: a revolution in clinical microbiology? Clinical Chemistry and Laboratory Medicine, v. 51, n. 2, p. 257-270, feb. 2013.

MARTINY, D. et al. Accuracy of the API Campy system, the Vitek 2 Neisseria-Haemophilus card and matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the identification of Campylobacter and related organisms. Clinical Microbiology and Infection, v. 17, n. 7, p. 1001-1006, jul. 2011.

MATHER, C. A.; RIVERA, S. F.; BUTLER-WU, S. M. Comparison of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry systems for identification of Mycobacteria using simplified protein extraction protocols. Journal of Clinical Microbiology, v. 52, n. 1, p. 130-138, jan. 2014.

MATTHIESEN, R.; MUTENDA, K. E. Introduction to Proteomics. In: MATTIESEN, R. Methods in Molecular Biology - Mass Spectrometry Data Analysis in Proteomics. New Jersey: Humana Press Inc., v. 367, 2007. p. 1-35

MELLMANN, A. et al. Evaluation of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. Journal of Clinical Microbiology, v. 46, n. 6, p. 1946-1954, jun. 2008.

MONTEIRO, J. et al. Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the Vitek® MS system. Journal of Microbiological Methods, v. 109, n. x, p. 157-159, feb. 2015.

PAILHORIÈS, H. et al. A case report of Mycoplasma hominis brain abscess identified by MALDI-TOF mass spectrometry. International Journal of Infectious Diseases, v. 29, n. x, p. 166-168, dec. 2014.

PATEL, R. MALDI-TOF MS for the diagnosis of infectious diseases. Clinical Chemistry, v. 61, n. 1, p. 100-111, jan. 2015.

PEREIRA, D. R. B. et al. Comparative analysis of two-dimensional electrophoresis maps (2-DE) of Helicobacter pylori from Brazilian patients with chronic gastritis and duodenal ulcer: a preliminary report. Revista do Instituto de Medicina Tropical de São Paulo, v. 48, n. 3, p. 175-177, may./jun. 2006.

PIGNONE, M. et al. Identification of Mycobacteria by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry. Journal of Clinical Microbiology, v. 44, n. 6, p. 1963-1970, jun. 2006.

PINTO, T. C. A. et al. Potential of MALDI-TOF MS as an alternative approach for capsular typing Streptococcus pneumoniae isolates. Scientific Reports, v. 28, n. 7, p. 45572, mar. 2017.

RAMOS, A. C. et al. Influence of culture media on detection of carbapenem hydrolysis by MALDI-TOF MS. Journal of Clinical Microbiology, v. 54, n. 7, p. 1896-1898, jul. 2016.

RODRIGUES, E. R. et al. Characterization of Achromobacter species in cystic fibrosis patients: comparison of blaOXA-114 PCR amplification, Multilocus Sequence Typing, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry. Journal of Clinical Microbiology, v. 53, n. 12, p. 3894-3896, dec. 2015.

SABBADINI, P. S. et al. Corynebacterium diphtheriae 67-72p hemagglutinin, characterized as the protein DIP0733, contributes to invasion and induction of apoptosis in HEp-2 cells. Microbial Pathogenesis, v. 52, n. 3, p. 165-176, mar. 2012.

SAFFERT, R. T. et al. Comparison of Bruker Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometer to BD Phoenix automated microbiology system for identification of Gram-negative bacilli. Journal of Clinical Microbiology, v. 49, n. 3, p. 887-892, mar. 2011.

SAICHEK, N. R. et al. Strain-level Staphylococcus differentiation by CeO2-metal oxide laser ionization mass spectrometry fatty acid profiling. BMC Microbiology, v. 16, n. x, p. 72, apr. 2016.

SCHAUMANN, R. et al. A step towards the discrimination of beta-lactamase-producing clinical isolates of Enterobacteriaceae and Pseudomonas aeruginosa by MALDI-TOF mass spectrometry. Medical Science Monitor, v. 18, n. 9, p. 71-77, sep. 2012.

SENG, P. et al. Ongoing revolution in Bacteriology: routine identification of bacteria by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry. Clinical Infectious Diseases, v. 49, n. 4, p. 543-551, aug. 2009.

SPARBIER, K. et al. Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-based functional assay for rapid detection of resistance against β-lactam antibiotics. Journal of Clinical Microbiology, v. 50, n. 3, p. 927-937, mar. 2012.

STEVENSON, L. G.; DRAKE, S. K.; MURRAY, P. R. Rapid identification of bacteria in positive blood culture broths by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Journal of Clinical Microbiology, v. 48, n. 2, p. 444-447, feb. 2010.

TANAKA, T. et al. Bactec™ blood culture bottles allied to MALDI-TOF mass spectrometry: rapid etiologic diagnosis of bacterial endophthalmitis. Diagnostic Microbiology and Infectious Disease, v. 88, n. 3, p. 222-224, jul. 2017.

VIEIRA, M. L. et al. Proteome analysis of Leptospira interrogans virulent strain. The Open Microbiology Journal, v. 7, n. 3, p. 69-74, may. 2009.

WERNO, A. M. et al. Differentiation of Streptococcus pneumoniae from nonpneumococcal streptococci of the Streptococcus mitis group by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Journal of Clinical Microbiology, v. 50, n. 9, p. 2863-2867, sep. 2012.

WIESER, A. et al. MALDI-TOF MS in microbiological diagnostics-identification of microorganisms and beyond (mini review). Applied Microbiology and Biotechnology, v. 93, n. 3, p. 965-974, feb. 2012.

WILEN, C. B.; McMULLEN, A. R.; BURNHAM, C.-A. D. Comparison of sample preparation methods, instrumentation platforms, and contemporary commercial databases for identification of clinically relevant Mycobacteria by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry. Journal of Clinical Microbiology, v. 53, n. 7, p. 2308-2315, jul. 2015.

Publicado
2018-10-15
Como Citar
1.
Pellegrino FLPC, Chagas TPG, Alves MS, Carvalho-Assef APD, Chapeaurouge A, Asensi MD. Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Applications in Bacteriology: brazilian contributions. hu rev [Internet]. 15º de outubro de 2018 [citado 15º de agosto de 2020];43(3):285 -294. Disponível em: https://periodicos.ufjf.br/index.php/hurevista/article/view/2859
Seção
Artigos de Revisão da Literatura