Efficiency of rLb6H recombinant protein from Leishmania (Viannia) braziliensis for the detection of canine visceral leishmaniasis

Efficacy of rLb6H in CVL diagnosis

Autores

  • Ingrid Estevam Pereira Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora https://orcid.org/0009-0007-5484-198X
  • Erick Esteves Oliveira Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora
  • Carolina Martins Moreira Elias Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora https://orcid.org/0000-0002-7010-8514
  • Alexandre Barbosa Reis Escola de Farmácia, Universidade Federal de Ouro Preto https://orcid.org/0000-0001-8123-4164
  • Malcolm Scott Duthie HDT Biocorp https://orcid.org/0000-0002-8515-5516
  • Henrique Couto Teixeira Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora

DOI:

https://doi.org/10.34019/1982-8047.2023.v49.41177

Palavras-chave:

Leishmaniose Canina, Diagnóstico, Proteínas Recombinantes, ELISA, rLb6H

Resumo

Introduction: Visceral leishmaniasis (VL) is a serious endemic disease in many tropical and subtropical countries, with a strong incidence in Brazil. The disease is transmitted by the bite of infected female phlebotomine sandflies, with dogs being the main urban reservoirs of the parasite. The diverse clinical profile and the long incubation period are challenges for the diagnosis of canine visceral leishmaniasis (CVL). Recombinant proteins from Leishmania spp. have been studied as antigens that can increase the accuracy of serological tests. Objective: To evaluate the diagnostic performance of the recombinant protein rLb6H, from Leishmania braziliensis, in comparison to the reference antigens rK39 and rK28, from L. donovani, prioritizing the identification of subclinical infected dogs. Material and Methods: Serum IgG reactivity to rLb6H, rK28, and rK39 recombinant proteins was assessed in dogs with previously parasitological confirmation of CVL, subdivided according to their clinical status, using immunoenzymatic assay (ELISA). Diagnostic accuracy of each ELISA was evaluated by receiver operating characteristic (ROC) curve analysis. Results: While all antigens showed a better performance in detecting CVL in symptomatic dogs (SD), detection of CVL in the oligosymptomatic (OD) and asymptomatic (AD) groups was lower, but rLb6H achieved high sensitivity for asymptomatic CVL. Interestingly, the most reactive CVL samples to rK28 were barely detected by rLb6H, while the less reactive to rK28, mostly from the AD group, presented higher reactivity to rLb6H. Conclusion: The recombinant protein rLb6H showed utility in the detection of asymptomatic CVL, displaying a complementary reactivity to rK39 and rK28. Thus, these results suggest that rLb6H could be incorporated into multi-antigen strategies, to increase diagnostic accuracy of CVL.

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Biografia do Autor

Henrique Couto Teixeira, Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora

Laboratório de Imunologia
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora
Tel/Fax: 32-2102-3214

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Publicado

2024-01-29

Como Citar

1.
Estevam Pereira I, Esteves Oliveira E, Martins Moreira Elias C, Barbosa Reis A, Scott Duthie M, Teixeira HC. Efficiency of rLb6H recombinant protein from Leishmania (Viannia) braziliensis for the detection of canine visceral leishmaniasis: Efficacy of rLb6H in CVL diagnosis. HU Rev [Internet]. 29º de janeiro de 2024 [citado 13º de abril de 2024];49:1-8. Disponível em: https://periodicos.ufjf.br/index.php/hurevista/article/view/41177

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