Proteômica: uma introdução aos métodos e aplicações

Autores

  • Nayara Braga Emidio Universidade Federal de Juiz de Fora http://orcid.org/0000-0001-7835-9636
  • Arthur Girardi Carpanez Universidade Federal de Juiz de Fora
  • Leonardo Ramos Quellis Universidade Federal de Juiz de Fora
  • Priscila Silva Farani Universidade Federal de Juiz de Fora
  • Eveline Gomes Vasconcelos Universidade Federal de Juiz de Fora
  • Priscila Faria-Pinto Universidade Federal de Juiz de Fora http://orcid.org/0000-0002-9011-2027

Palavras-chave:

Proteômica, Espectrometria de Massas, Peptídeos, Proteínas, Biomarcadores

Resumo

As proteínas desempenham a maior parte das funções fisiológicas das células, constituindo também importantes alvos farmacológicos e biomarcadores de doenças. A pesquisa qualitativa, quantitativa e a elucidação estrutural destas moléculas são fundamentais para a compreensão do funcionamento dos sistemas biológicos, bem como na aplicação destas para o desenvolvimento de novos métodos diagnóstico. O estudo do proteoma nos permite identificar as proteínas que estão sendo expressas em um determinado momento, quantificá-las e observar suas modificações pós-transducionais. Dessa maneira, a análise proteômica fornece informações mais abrangentes e que não podem ser inferidas a partir das informações obtidas através da análise genômica. Este tipo de estudo envolve etapas como: extração e tratamento da amostra, separação das proteínas e/ou peptídeos, espectrometria de massas e análise dos dados usando ferramentas de bioinformática. O presente trabalho faz uma revisão narrativa sobre as principais técnicas aplicadas desde o preparo de amostras até a identificação das proteínas. 

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Biografia do Autor

Nayara Braga Emidio, Universidade Federal de Juiz de Fora

Laboratório de Estrutura e Função de Proteínas/ Departamento de Bioquímica/Instituto de Ciências Biológicas

Arthur Girardi Carpanez, Universidade Federal de Juiz de Fora

Departamento de Química/Instituto de Ciências Exatas

Leonardo Ramos Quellis, Universidade Federal de Juiz de Fora

Laboratório de Estrutura e Função de Proteínas/ Departamento de Bioquímica/Instituto de Ciências Biológicas

Priscila Silva Farani, Universidade Federal de Juiz de Fora

Laboratório de Estrutura e Função de Proteínas/ Departamento de Bioquímica/Instituto de Ciências Biológicas

Eveline Gomes Vasconcelos, Universidade Federal de Juiz de Fora

Laboratório de Estrutura e Função de Proteínas/ Departamento de Bioquímica/Instituto de Ciências Biológicas

Priscila Faria-Pinto, Universidade Federal de Juiz de Fora

Laboratório de Estrutura e Função de Proteínas/ Departamento de Bioquímica/Instituto de Ciências Biológicas

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2016-04-19

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Braga Emidio N, Girardi Carpanez A, Ramos Quellis L, Silva Farani P, Gomes Vasconcelos E, Faria-Pinto P. Proteômica: uma introdução aos métodos e aplicações. hu rev [Internet]. 19º de abril de 2016 [citado 12º de agosto de 2022];41(3 e 4). Disponível em: https://periodicos.ufjf.br/index.php/hurevista/article/view/2482

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