Morphological and Morphometric Characterization of Trypanosomes in Leptodactylus lineatus and Osteocephalus sp. (Anura) from Brazilian Midwest

  • André Flávio Soares Ferreira Rodrigues Departamento de Ciências Naturais - CDB, Universidade Federal de São João del-Rei. Pça D. Helvécio n. 74, Fábricas, São João del-Rei, MG, Brazil http://orcid.org/0000-0001-5135-7331
  • Drausio Honório Morais Universidade Federal Rural da Amazônia (UFRA), PA 275, Km 13, Zona Rural. Parauapebas, Pará, Brazil https://orcid.org/0000-0002-9866-6008
  • Vinícius Tadeu Carvalho Programa de Pós-Graduação em Bioprospecção Molecular, Universidade Regional do Cariri, Campus do Pimenta, Rua Cel. Antônio Luiz, Crato, CE, Brazil
  • Marta D’Agosto Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n - Campus Universitário Martelos, Bairro São Pedro, 36036-000 Juiz de Fora, MG, Brazil
  • Moara Lemos Département de Parasites et Insectes Vecteurs, Institut Pasteur, Rue Dr. Roux 25-28, Paris, France https://orcid.org/0000-0002-7986-9053
Palavras-chave: Amazon Forest, Anurans, Cerrado, Trypanosoma

Resumo

The objective of this study was to evaluate parasitism caused by trypanosomes in naturally infected anurans from Brazilian Midwest and characterize trypanosomes by morphology and morphometry. Anurans were captured from margins of the Guaporé River in transition areas between Cerrado and Rain Forest. Blood samples of 16 anurans species were obtained by cardiac puncture and analyzed for the presence of hemoparasites. Trypanosomes were found infecting two anuran species, Leptodactylus lineatus (Leptodactylidae) and Osteocephalus sp. (Hylidae). It was observed high prevalence of trypanosomes (100%; N = 3) in L. lineatus, with intensity of infection of 9.9 × 102 parasites/ml. In Osteocephalus sp. the prevalence of trypanosomes was comparatively lower (36%; N = 4 from 11), however, the intensity of infection was higher 2.16 × 103 parasites/ml. Morphological variation related to body length and width of bloodstream trypomastigotes were observed in different host species and between individual hosts of the same species. Polymorphic trypanosomes were separated into morphotypes and classified as elongated or short trypanosomes. Trypanosomes infecting L. lineatus are elongated with slender or broad body and trypanosomes infecting Osteocephalus sp. presented a short body with broad posterior extremity. The parasitological analyses and morphological characterizations presented in this study contribute to the knowledge of diversity of trypanosomes from neotropical anurans.

Publicado
2019-08-26